Investigando cinza da casca do arroz como fase estacionária em cromatografia: uma proposta de aula experimental nos cursos de graduação

Química Nova

João R. De Freitas Filho²  Jucleiton J. Rufino De Freitas²  Ladjane Pereira Da Silva²  Juliano C. Rufino Freitas¹ 

[1] ,Universidade Federal de Rural de Pernambuco Departamento de Química Recife PE ,Brasil

关键词: rice husk ash;    stationary phase;    chromatography;   

DOI  :  10.1590/S0100-40422012000200033

来源: SciELO

【 摘 要 】

This article describes an experiment that involves the extraction and separation of the pigments of spinach extract by column chromatography using the silica obtained from rice husk ash as a stationary phase. The experimental procedure is very simple to apply in the chemistry laboratory, and allowed to illustrate some concepts of organic chemistry: structure of organic molecules, solubility, polarity, extraction, partition coefficient, adsorption and retention factor (Rf), as well as objectives and fundamentals of chromatographic methods. The experiment can be carried out in a 4 h period.

【 授权许可】

CC BY-NC   
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