The investigation rice husk ash as a stationary phase in chromatography: a proposal of experimental class in undergraduate courses

Química Nova

Freitas Filho, João R. de¹  Freitas, Juliano C. Rufino¹  Silva, Ladjane Pereira da¹  Freitas, Jucleiton J. Rufino de¹  Universidade Federal de Pernambuco, Recife, Brasil¹  Universidade Federal de Rural de Pernambuco, Recife, Brasil¹ 

关键词: rice husk ash;    stationary phase;    chromatography.;   

DOI  :  10.1590/S0100-40422012000200033

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

This article describes an experiment that involves the extraction and separation of the pigments of spinach extract by column chromatography using the silica obtained from rice husk ash as a stationary phase. The experimental procedure is very simple to apply in the chemistry laboratory, and allowed to illustrate some concepts of organic chemistry: structure of organic molecules, solubility, polarity, extraction, partition coefficient, adsorption and retention factor (Rf), as well as objectives and fundamentals of chromatographic methods. The experiment can be carried out in a 4 h period.

【 授权许可】

Unknown