Derivative spectrophotometric method for determination of acyclovir in polymeric nanoparticles

Química Nova

Guilherme Diniz Tavares¹  Gisele Miki Ishikawa¹  Talita Ferreira Monteiro¹  Carolina Zanolini¹  Érika Rosa Maria Kedor-hackmann¹  Nádia Araci Bou-chacra¹  Vladi Olga Consiglieri¹ 

[1] ,Universidade de São Paulo Faculdade de Ciências Farmacêuticas São Paulo SP ,Brasil

关键词: acyclovir;    polymeric nanoparticles;    derivative spectrophotometry;   

DOI  :  10.1590/S0100-40422012000100035

来源: SciELO

【 摘 要 】

A derivative spectrophotometric method was validated for quantification of acyclovir in poly (n-butylcyanoacrylate) (PBCA) nanoparticles. Specificity, linearity, precision, accuracy, recovery, detection (LOD) and quantification (LOQ) limits were established for method validation. First-derivative at 295.2 nm eliminated interferences from nanoparticle ingredients and presented linearity for acyclovir concentrations ranging from 1.25 to 40.0 µg/mL (r = 0.9999). Precision and accuracy data demonstrated good reproducibility. Recovery ranged from 99.3 to 101.2. LOD was 0.08 µg/mL and LOQ, 0.25 µg/mL. Thus, the proposed method proved to be easy, low cost, and accurate, and therefore, an useful alternative to quantify acyclovir in nanoparticles.

【 授权许可】

CC BY-NC   
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