Otimização e validação de método multirresíduo para determinação de sulfonamidas em camarão cultivado por cromatografia líquida de alta eficiência com detecção por UV

Química Nova

Paloma Nascimento Dos Santos²  Maria Inês Sucupira Maciel²  Beatriz Regina Brito De Oliveira Lavorante¹  Marcelo De Mélo Medeiros¹  Eden Cavalcanti De Albuquerque Júnior¹ 

[1] ,Universidade Federal Rural de Pernambuco Departamento de Química Recife PE ,Brasil

关键词: sulfonamides;    shrimp;    HPLC-UV;   

DOI  :  10.1590/S0100-40422011000700027

来源: SciELO

【 摘 要 】

This work reports the optimization and method validation for sulfonamides (sulfamethazine, sulfaquinoxaline, sulfadimethoxine and sulfathiazole) in shrimp muscle using HPLC-UV. The sulfonamides were extracted with acetonitrile and acetic acid, and the extract cleaned up with a Strata SCX SPE cartridge prior to analysis. The method presented linearity in the range of 20-120 µg kg-1, good linear correlation (r > 0.99), and limits of quantification in the range of 4.7-20.2 µg kg-1. The recovery for shrimp muscles spiked with 50-150 µg kg-1 ranged from 63.2-108.0%. Precision and accuracy analysis showed acceptable relative standard deviation. Commercial shrimps were analyzed and sulfonamides don't were found above of the method limit of quantification.

【 授权许可】

CC BY-NC   
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