Optimization and validation multiresidue method for sulfonamides determination in shrimp by high performance liquid chromatography with detection by UV

Quimica nova

Albuquerque Júnior, Eden Cavalcanti de¹  Lavorante, Beatriz Regina Brito de Oliveira¹  Medeiros, Marcelo de Mélo¹  Santos, Paloma Nascimento dos²  Maciel, Maria Inês Sucupira² 

[1] Instituto de Tecnologia de Pernambuco, Recife, Brasil;Universidade Federal Rural de Pernambuco, Recife, Brasil

关键词: sulfonamides;    shrimp;    HPLC-UV.;   

DOI  :  10.1590/S0100-40422011000700027

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

This work reports the optimization and method validation for sulfonamides (sulfamethazine, sulfaquinoxaline, sulfadimethoxine and sulfathiazole) in shrimp muscle using HPLC-UV. The sulfonamides were extracted with acetonitrile and acetic acid, and the extract cleaned up with a Strata SCX SPE cartridge prior to analysis. The method presented linearity in the range of 20-120 µg kg-1, good linear correlation (r > 0.99), and limits of quantification in the range of 4.7-20.2 µg kg-1. The recovery for shrimp muscles spiked with 50-150 µg kg-1 ranged from 63.2-108.0%. Precision and accuracy analysis showed acceptable relative standard deviation. Commercial shrimps were analyzed and sulfonamides don't were found above of the method limit of quantification.

【 授权许可】

CC BY