Determinação de citrato de sildenafila e de tadalafila por cromatografia líquida de ultraeficiência com detecção por arranjo de diodos (CLUE-DAD)

Química Nova

Rafael Scorsatto Ortiz²  Marina Venzon Antunes¹  Rafael Linden¹ 

[1] ,Superintendência de Polícia Federal no Estado do Rio Grande do SulPorto Alegre RS ,Brasil

关键词: sildenafil;    tadalafil;    ultra performance liquid chromatography;   

DOI  :  10.1590/S0100-40422010000200028

来源: SciELO

【 摘 要 】

A simple ultra-performance liquid chromatographic method for the simultaneous determination of sildenafil citrate and tadalafil was developed and validated. Sample preparation was dissolution in methanol, followed by centrifugation and dilution (1:10) with methanol. Analysis was performed in an Acquity® UPLC system with Acquity® BEH C18 column (2.1 x 50 mm, with 1.7 μm particles). The elution was isocratic with phosphate buffer pH 2.3 and acetonitrile (65:35, v/v) at a flow rate of 0.7 mL/min. The method presented adequate specificity, linearity, precision and accuracy and allowed the determination of the drugs in seized forensic samples.

【 授权许可】

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