Determination of sildenafil citrate and tadalafil by ultra performance liquid chromatography with diode-array detection (UPLC-DAD)

Química Nova

Linden, Rafael¹  Centro Universitário Feevale, Novo Hamburgo, Brasil¹  Antunes, Marina Venzon¹  Ortiz, Rafael Scorsatto¹  Superintendência de Polícia Federal no Estado do Rio Grande do Sul, Porto Alegre, Brasil¹ 

关键词: sildenafil;    tadalafil;    ultra performance liquid chromatography.;   

DOI  :  10.1590/S0100-40422010000200028

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A simple ultra-performance liquid chromatographic method for the simultaneous determination of sildenafil citrate and tadalafil was developed and validated. Sample preparation was dissolution in methanol, followed by centrifugation and dilution (1:10) with methanol. Analysis was performed in an Acquity® UPLC system with Acquity® BEH C18 column (2.1 x 50 mm, with 1.7 μm particles). The elution was isocratic with phosphate buffer pH 2.3 and acetonitrile (65:35, v/v) at a flow rate of 0.7 mL/min. The method presented adequate specificity, linearity, precision and accuracy and allowed the determination of the drugs in seized forensic samples.

【 授权许可】

Unknown