Química Nova | |
O estado da arte da cromatografia líquida de ultra eficiência | |
Liane Maldaner1  Isabel Cristina Sales Fontes Jardim1  | |
[1] ,Universidade Estadual de Campinas Instituto de Química Departamento de Química AnalíticaCampinas SP ,Brasil | |
关键词: ultra performance liquid chromatography; sub-2 µm particles; fast analysis; | |
DOI : 10.1590/S0100-40422009000100036 | |
来源: SciELO | |
【 摘 要 】
Ultra Performance Liquid Chromatography (UPLC) retains the same principles as High Performance Liquid Chromatography (HPLC), but uses 1-2.1 mm i.d. columns with sub-2 µm particles. It is considered the newest advance in analytical separation science. The use of these small particles with mobile phases at high linear velocities increases resolution and detectability and decreases analysis time. Thus, the analyses are faster, the solvent volume is smaller, the efficiency is higher and the detectability is 2-3 times higher when compared with HPLC analysis.
【 授权许可】
CC BY-NC
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
【 预 览 】
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