Heavy atom enhanced room-temperature phosphorimetry for ultratrace determination of harmane

Química Nova

Flávia F. De Carvalho Marques¹  Flávia Da Silva Figueiredo¹  Ricardo Queiroz Aucelio¹ 

[1] ,Pontifícia Universidade Católica do Rio de Janeiro Departamento de Química Rio de Janeiro RJ ,Brazil

关键词: room-temperature phosphorescence;    harmane;    selective heavy atom effect;   

DOI  :  10.1590/S0100-40422008000200003

来源: SciELO

【 摘 要 】

Harmane has been proposed for the treatment of epilepsy, AIDS and leshmaniosis. Its room-temperature phosphorescence was induced using either AgNO3 or TlNO3, enabling absolute limits of detection of 0.12 and 2.4 ng respectively, with linear dynamic ranges extending up to 456 ng (AgNO3) and 911 ng (TlNO3). Relative standard deviations around 3% were observed for substrates containing 46 ng of harmane. Such sensitivity and precision are needed because harmane intake must be strictly controlled to achieve proper therapeutic response. Interference studies were performed using thalidomide, reserpine and yohimbine. Recovery of 104±6% was achieved using solid surface room-temperature phosphorimetry. The result was comparable to the one obtained by micellar electrokinetic chromatography.

【 授权许可】

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