Heavy atom enhanced room-temperature phosphorimetry for ultratrace determination of harmane

Quimica nova

Aucelio, Ricardo Queiroz¹  Figueiredo, Flávia da Silva¹  Marques, Flávia F. de Carvalho¹ 

[1] Pontifícia Universidade Católica do Rio de Janeiro, Rio de Janeiro, Brazil

关键词: room-temperature phosphorescence;    harmane;    selective heavy atom effect.;   

DOI  :  10.1590/S0100-40422008000200003

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Harmane has been proposed for the treatment ofepilepsy, AIDS and leshmaniosis. Its room-temperature phosphorescence was inducedusing either AgNO3 or TlNO3, enabling absolutelimits of detection of 0.12 and 2.4 ng respectively, with linear dynamic rangesextending up to 456 ng (AgNO3) and 911 ng (TlNO3). Relativestandard deviations around 3% were observed for substrates containing 46 ngof harmane. Such sensitivity and precision are needed because harmane intakemust be strictly controlled to achieve proper therapeutic response. Interferencestudies were performed using thalidomide, reserpine and yohimbine. Recoveryof 104±6% was achieved using solid surface room-temperature phosphorimetry.The result was comparable to the one obtained by micellar electrokinetic chromatography.

【 授权许可】

CC BY