Determinação espectrofotométrica de vitamina B2 (riboflavina) em formulações farmacêuticas empregando sistema de análises por injeção em fluxo

Química Nova

Clezio Aniceto¹  Larissa De Souza Canaes¹  Orlando Fatibello-filho¹  Carla C. S. Cavalheiro² 

[1] ,Universidade Federal de São Carlos Centro de Ciências Exatas e de Tecnologia Departamento de QuímicaSão Carlos SP

关键词: vitamin B2;    flow injection spectrophotometry;    pharmaceutical preparations;   

DOI  :  10.1590/S0100-40422000000500013

来源: SciELO

【 摘 要 】

A flow injection spectrophotometric procedure exploiting merging zones is proposed for determining vitamin B2 (riboflavin) in pharmaceutical preparations. The determination is based on the red-colored complex formation between vitamin B2 and silver(I) which was measured at 520 nm. Vitamin B2 was determined in four pharmaceutical preparations in the 1.0 to 50.0 mg L-1 concentration range, with a detection limit of 0.5 mg L-1. The recovery from three samples ranged from 98.0 to 104.0 %. The analytical frequency was 42 h-1 and r.s.d. were lower than 1% for solutions containing 10.0, 30.0 and 50.0 mg L-1 vitamin B2 (n= 10). The results obtained in commercial formulations using the FIA procedure were in good agreement with those obtained by using a conventional fluorimetric procedure (r=0.9998) and also with the label values (r= 0.9997).

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License