Polymorphism of the grasshopper Rhammatocerus schistocercoides populations revealed by RAPD

Pesquisa Agropecuária Brasileira

João Batista Tavares Da Silva¹  Myrian Silvana Tigano¹  Bonifácio Peixoto Magalhães¹  Célia Maria Torres Cordeiro¹ 

[1] ,Embrapa Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia Brasília DF

关键词: Insecta;    genetic marker;    genetic variation;    genetic distance;    Insecta;    marcador genético;    variação genética;    distância genética;   

DOI  :  10.1590/S0100-204X2002001100020

来源: SciELO

【 摘 要 】

The objective of this work was to study the genetic variability of the grasshopper Rhammatocerus schistocercoides (Orthoptera: Acrididae) using RAPD analysis among individuals from three populations, one from Colombia and two from Brazil (Goiás and Mato Grosso States). Ninety scorable binary markers were obtained by fingerprinting with 11 oligonucleotide primers. Most of the polymorphism was attributed to 42 markers with variable frequency among the different populations. Although the existence of significant difference among populations (P<0.0001), most of the genetic variability was found within populations (87.7% of total variation). Pairwise distances between Colombian and Brazilian populations were 0.12 (P<0.0001) and 0.18 (P<0.0001) for Goiás and Mato Grosso, respectively. The pairwise distance between Goiás and Mato Grosso populations was 0.06 (P<0.0001). These data indicated that the phenotypic differences among populations are associated mainly with the geographical distances between the Brazilian and Colombian populations.

【 授权许可】

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