Genetic Diversity of Red Chittagong Cattle Using Randomly Amplified Polymorphic DNA Markers| Science Publications

American Journal of Animal and Veterinary Sciences

Mohammad M.R Mufti¹  Gautam K. Deb¹  K. Nahar¹  Mst P. Mostari¹  Khan S. Huque¹ 

关键词: Red chittagong cattle;    RAPD;    PCR;    genetic variation;    genetic distance;   

DOI  :  10.3844/ajavsp.2009.1.5

学科分类：兽医学

来源: Science Publications

【 摘 要 】

Problem statement: Red Chittagong Cattle (RCC) is a potential genetic resource in Bangladesh having dairy potentialities, high resistance to disease and survive harse environmental condition while require low input supply. This resource is now disappearing due to indiscriminate crossing with other local and exotic cattle. To conserve this resource, find out the genetic diversity of RCC is prime concern. Approach: To determine the genetic variation of Red Chittagong Cattle and to find out the genetic present status for their future improvement and conservation program. The experiment was conducted in the Molecular Genetics Laboratory under Animal Production Research Division, Bangladesh Livestock Research Institute (BLRI), Saver, Dhaka, Bangladesh. DNA was extracted from 100 RCC blood samples and the extracted DNA was observed by gel electrophoresis. Randomly 16 primers were tested among them three primers were matched and found polymorphic. Random Amplification of Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) analysis was carried out using DNA samples of RCC. Only unambiguous, reproducible and scorable polymorphic fragments were taken into consideration for analysis. Data were analyzed by using a computer program POPGENE (Version 1.31). Results: The highest and the lowest level of gene frequency value (0.9590 and 0.0440) were observed in allele 0 and 1 with BMC1222-2 locus and only one monomorphic locus (OPB07-1) was found. The Nei

【 授权许可】

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