Establishment of an in vitro system for studies on the induced resistance of cotton to Xanthomonas campestris pv. malvacearum

Pesquisa Agropecuária Brasileira

Adilson Kenji Kobayashi¹  Luiz Gonzaga Esteves Vieira¹ 

[1] ,Instituto Agronômico do ParanáLondrina PR

关键词: Gossypium hirsutum;    bacterial blight;    extracellular polysaccharides;    cell suspension culture;    hypersensitive response;    Gossypium hirsutum;    bacteriose;    polissacarídeos extracelulares;    cultura de células em suspensão;    hipersensibilidade;   

DOI  :  10.1590/S0100-204X2000000400007

来源: SciELO

【 摘 要 】

An in vitro system for studying the resistance response of cotton (Gossypium hirsutum L.) to Xanthomonas campestris pv. malvacearum was investigated. Cell suspension cultures, established from hypocotyl-derived callus of cotton cultivar 101-102B, were treated with bacterial extracellular polysaccharides (EPS) extracted from the incompatible race 18 of X. campestris pv. malvacearum. EPS at 600 mug/mL caused pronounced darkening of the suspension cultures, as indicative of cell death, 48 hours after incubation. Protein electrophoresis analysis of the time course of EPS-treated cells showed differential accumulation of several protein bands after 12-24 hours. The time course of protein accumulation and cell death was consistent with an elicitor-mediated hypersensitive response.

【 授权许可】

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