Mutation Breeding of Extracellular Polysaccharide-Producing Microalga Crypthecodinium cohnii by a Novel Mutagenesis with Atmospheric and Room Temperature Plasma

International Journal of Molecular Sciences

Bin Liu²  Zheng Sun¹  Xiaonian Ma¹  Bo Yang²  Yue Jiang³  Dong Wei²  Feng Chen¹ 

[1]Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China

[2] E-Mails:

[3]School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China

[4] E-Mails:

[5]School of Food Science, Jiangnan University, Wuxi 214122, China

[6] E-Mail:

关键词: extracellular polysaccharides;    atmospheric and room temperature plasma (ARTP);    mutation;    microalgae;    Crypthecodinium cohnii;   

DOI  :  10.3390/ijms16048201

来源: mdpi

【 摘 要 】

Extracellular polysaccharides (EPS) produced by marine microalgae have the potential to be used as antioxidants, antiviral agents, immunomodulators, and anti-inflammatory agents. Although the marine microalga Crypthecodinium cohnii releases EPS during the process of docosahexaenoic acid (DHA) production, the yield of EPS remains relatively low. To improve the EPS production, a novel mutagenesis of C. cohnii was conducted by atmospheric and room temperature plasma (ARTP). Of the 12 mutants obtained, 10 mutants exhibited significantly enhanced EPS yield on biomass as compared with the wild type strain. Among them, mutant M7 was the best as it could produce an EPS volumetric yield of 1.02 g/L, EPS yield on biomass of 0.39 g/g and EPS yield on glucose of 94 mg/g, which were 33.85%, 85.35% and 57.17% higher than that of the wild type strain, respectively. Results of the present study indicated that mutagenesis of the marine microalga C. cohnii by ARTP was highly effective leading to the high-yield production of EPS.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.