Tuberculin skin test and interferon-gamma release assay values are associated with antimicrobial peptides expression in polymorphonuclear cells during latent tuberculous infection

Memórias do Instituto Oswaldo Cruz

Julio E Castañeda-delgado¹  Alberto Cervantes-villagrana¹  Carmen J Serrano-escobedo¹  Isabel Frausto-lujan¹  Cesar Rivas-santiago¹  Jose A Enciso-moreno¹  Bruno Rivas-santiago¹ 

关键词: tuberculosis;    latent infection;    antimicrobial peptides;    tuberculin skin test;    QuantiFERON;    biomarker;   

DOI  :  10.1590/0074-0276140348

来源: SciELO

【 摘 要 】

It has been reported that patients with progressive tuberculosis (TB) express abundant amounts of the antimicrobial peptides (AMPs) cathelicidin (LL-37) and human neutrophil peptide-1 (HNP-1) in circulating cells, whereas latent TB infected donors showed no differences when compared with purified protein derivative (PPD) and QuantiFERON®-TB Gold (QFT)-healthy individuals. The aim of this study was to determine whether LL-37 and HNP-1 production correlates with higher tuberculin skin test (TST) and QFT values in TB household contacts. Twenty-six TB household contact individuals between 26-58 years old TST and QFT positive with at last two years of latent TB infection were recruited. AMPs production by polymorphonuclear cells was determined by flow cytometry and correlation between TST and QFT values was analysed. Our results showed that there is a positive correlation between levels of HNP-1 and LL-37 production with reactivity to TST and/or QFT levels. This preliminary study suggests the potential use of the expression levels of these peptides as biomarkers for progression in latent infected individuals.

【 授权许可】

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