Memórias do Instituto Oswaldo Cruz | |
Mycobacterium leprae virulence-associated peptides are indicators of exposure to M. leprae in Brazil, Ethiopia and Nepal | |
Kidist Bobosha2  Sheila Tuyet Tang1  Jolien J Van Der Ploeg-van Schip2  Yonas Bekele1  Marcia Vsb Martins1  Ole Lund1  Kees Lmc Franken2  Saraswoti Khadge1  Maria Araci De Andrade Pontes1  Heitor De Sá Gonçalves1  Jemal Hussien1  Pratibha Thapa1  Chhatra B Kunwar1  Deanna A Hagge1  Abraham Aseffa1  Maria Cristina Vidal Pessolani1  Geraldo Mb Pereira1  Tom Hm Ottenhoff2  Annemieke Geluk2  | |
[1] ,University Medical Center Department of Infectious Diseases Leiden,The Netherlands | |
关键词: M. leprae; leprosy; biomarkers; bioinformatics; virulence; peptides; | |
DOI : 10.1590/S0074-02762012000900018 | |
来源: SciELO | |
【 摘 要 】
Silent transmission of Mycobacterium leprae, as evidenced by stable leprosy incidence rates in various countries, remains a health challenge despite the implementation of multidrug therapy worldwide. Therefore, the development of tools for the early diagnosis of M. leprae infection should be emphasised in leprosy research. As part of the continuing effort to identify antigens that have diagnostic potential, unique M. leprae peptides derived from predicted virulence-associated proteins (group IV.A) were identified using advanced genome pattern programs and bioinformatics. Based on human leukocyte antigen (HLA)-binding motifs, we selected 21 peptides that were predicted to be promiscuous HLA-class I T-cell epitopes and eight peptides that were predicted to be HLA-class II restricted T-cell epitopes for field-testing in Brazil, Ethiopia and Nepal. High levels of interferon (IFN)-γ were induced when peripheral blood mononuclear cells (PBMCs) from tuberculoid/borderline tuberculoid leprosy patients located in Brazil and Ethiopia were stimulated with the ML2055 p35 peptide. PBMCs that were isolated from healthy endemic controls living in areas with high leprosy prevalence (EChigh) in Ethiopia also responded to the ML2055 p35 peptide. The Brazilian EChigh group recognised the ML1358 p20 and ML1358 p24 peptides. None of the peptides were recognised by PBMCs from healthy controls living in non-endemic region. In Nepal, mixtures of these peptides induced the production of IFN-γ by the PBMCs of leprosy patients and EChigh. Therefore, the M. leprae virulence-associated peptides identified in this study may be useful for identifying exposure to M. leprae in population with differing HLA polymorphisms.
【 授权许可】
CC BY
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