An evaluation of p16INK4a expression in cervical intraepithelial neoplasia specimens, including women with HIV-1

Memórias do Instituto Oswaldo Cruz

Alcina F Nicol¹  Jonathan E Golub²  José R Lapa E Silva²  Cynthia B Cunha²  Sergio M Amaro-filho¹  Nathalia S Oliveira¹  Willker Menezes¹  Cecília Viana Andrade²  Fabio Russomano²  Aparecida Tristão²  Beatriz Grinsztejn²  Ruth Khalili Friedman²  Marcia P Oliveira¹  Andrea Pires²  Gerard J Nuovo² 

[1] ,Instituto Oswaldo Cruz Laboratório Interdisciplinar de Pesquisas Médicas

关键词: CIN;    p16INK4a;    HPV;    HIV;    TMA;    ROC;   

DOI  :  10.1590/S0074-02762012000500001

来源: SciELO

【 摘 要 】

Although several studies have evaluated the role of p16INK4a as a diagnostic marker of cervical intraepithelial neoplasia (CIN) and its association with disease progression, studies regarding the role of p16INK4a in human immunodeficiency virus (HIV)-infected patients remain scarce. The present study was designed to determine the potential utility of p16INK4a as a diagnostic marker for CIN and invasive cervical cancer in HIV-positive and negative cervical specimens. An immunohistochemical analysis of p16INK4a was performed in 326 cervical tissue microarray specimens. Performance indicators were calculated and compared using receiving operating characteristics curve (ROC)/area under the curve. In HIV-1-negative women, the percentage of cells that was positive for p16INK4a expression was significantly correlated with the severity of CIN (p < 0.0001). A ROC curve with a cut-off value of 55.28% resulted in a sensitivity of 89%, a specificity of 81%, a positive predictive value of 91% and a negative predictive value of 78%. HIV-seropositive women exhibited decreased expression of p16INK4a in CIN2-3 specimens compared with HIV-negative specimens (p = 0.031). The ROC data underscore the potential utility of p16INK4a under defined conditions as a diagnostic marker for CIN 2-3 staging and invasive cervical cancer. HIV-1 infection, however, is associated with relatively reduced p16INK4a expression in CIN 2-3.

【 授权许可】

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