Occurrence of blaOXA-23 gene in imipenem-susceptible Acinetobacter baumannii

Memórias do Instituto Oswaldo Cruz

Karyne Rangel Carvalho²  Ana Paula D'alincourt Carvalho-assef²  Lia Galvão Dos Santos¹  Maria José Félix Pereira¹  Marise Dutra Asensi² 

[1] ,Instituto Oswaldo Cruz-Fiocruz Laboratório de Pesquisa em Infecção Hospitalar Rio de Janeiro RJ ,Brasil

关键词: imipenem-susceptible;    Acinetobacter baumannii;    blaOXA-23;   

DOI  :  10.1590/S0074-02762011000400020

来源: SciELO

【 摘 要 】

The aim of the current study was to describe the occurrence of the blaOXA-23 gene and the ISAba1 element in imipenem-susceptible Acinetobacter baumannii strains. By performing the polymerase chain reaction mapping using combinations of ISAba1 forward primers and the blaOXA-23-like gene reverse primers, we demonstrated that the ISAba1 element did not occur upstream of the blaOXA-23 gene in five of 31 isolates, which explained the lack of resistance to imipenem despite the presence of the blaOXA-23 gene. All of the blaOXA-23-positive isolates were susceptible to imipenem and meropenem with minimal inhibitory concentration < 4 µg/mL. Pulsed-field gel electrophoresis analysis revealed four genotypes among the five blaOXA-23-positive isolates. The current report of the blaOXA-23 gene in imipenem-susceptible isolates provided evidence that this gene may be silently spread in a hospital environment and highlighted the threat of undetected reservoirs of carbapenemase genes.

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