Mycobacterium leprae downregulates the expression of PHEX in Schwann cells and osteoblasts

Memórias do Instituto Oswaldo Cruz

Sandra R Boiça Silva²  Antônio J Tempone¹  Tatiana P Silva¹  Maria Renata Sn Costa¹  Geraldo Mb Pereira²  Flávio A Lara¹  Maria Cristina V Pessolani¹  Danuza Esquenazi¹ 

[1] ,Universidade do Estado do Rio de Janeiro Faculdade de Ciências Médicas Laboratório de ImunopatologiaRio de Janeiro RJ ,Brasil

关键词: Mycobacterium leprae;    leprosy;    PHEX;    osteoblasts;    Schwann cells;   

DOI  :  10.1590/S0074-02762010000500005

来源: SciELO

【 摘 要 】

Neuropathy and bone deformities, lifelong sequelae of leprosy that persist after treatment, result in significant impairment to patients and compromise their social rehabilitation. Phosphate-regulating gene with homologies to endopeptidase on the X chromosome (PHEX) is a Zn-metalloendopeptidase, which is abundantly expressed in osteoblasts and many other cell types, such as Schwann cells, and has been implicated in phosphate metabolism and X-linked rickets. Here, we demonstrate that Mycobacterium leprae stimulation downregulates PHEX transcription and protein expression in a human schwannoma cell line (ST88-14) and human osteoblast lineage. Modulation of PHEX expression was observed to a lesser extent in cells stimulated with other species of mycobacteria, but was not observed in cultures treated with latex beads or with the facultative intracellular bacterium Salmonella typhimurium. Direct downregulation of PHEX by M. leprae could be involved in the bone resorption observed in leprosy patients. This is the first report to describe PHEX modulation by an infectious agent.

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