Detection of Lsr2 gene of Mycobacterium leprae in nasal mucus

Brazilian Archives of Biology and Technology

Luiz Antonio Custodio²  Alexandre Saito¹  Marla Karine Amarante²  Thiago Cezar Fujita¹  Aparecida De Lourdes Perim²  Ivete Conchon Costa¹  Ionice Felipe¹  Shiduca Itow Jankevicius¹ 

[1],Universidade Estadual de Londrina Centro de Ciências da Saúde Departamento de Patologia Aplicada, Análise Clínicas e Toxicológicas PR ,Brasil

关键词: Mycobacterium leprae;    leprosy;    hanseniase;    nasal mucus;    PCR;   

DOI  :  10.1590/S1516-89132012000300007

来源: SciELO

【 摘 要 】

In the present study, nasal mucus from patients with leprosy were analyzed by PCR using specific primers for Lsr2 gene of Mycobacterium leprae. The presence of Lsr2 gene in the nasal mucus was detected in 25.80% of patients with paucibacillari leprosy, and 23.07% of contacts. Despite the absence of clinical features in the contact individuals, it was possible to detect the presence of Lsr2 gene in the nasal mucus of these individuals. Therefore, PCR detection of M. leprae targeting Lsr2 gene using nasal mucus samples could contribute to early diagnosis of leprosy.

【 授权许可】

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