Antibody reactivity against potato apyrase, a protein that shares epitopes with Schistosoma mansoni ATP diphosphohydrolase isoforms, in acute and chronically infected mice, after chemotherapy and reinfection

Memórias do Instituto Oswaldo Cruz

Priscila De Faria-pinto²  Maria Ângela Montesano²  Alexandre Arthur Jacinto²  Ronaldo Soares Santos²  Fábio Humberto S Bordin²  Ana Paula Ferreira²  Marcus Luiz O Penido¹  Paulo Marcos Z Coelho¹  Eveline G Vasconcelos² 

[1] ,Universidade Federal de Juiz de Fora Instituto de Ciências Biológicas Departamento de Bioquímica

关键词: Schistosoma mansoni;    ATP diphosphohydrolase;    potato apyrase;    antibody;    BALB/c mouse;    chemotherapy;   

DOI  :  10.1590/S0074-02762010000400005

来源: SciELO

【 摘 要 】

Schistosoma mansoni ATP diphosphohydrolase isoforms and potato apyrase share conserved epitopes. By enzyme-linked immunosorbent assays, elevated levels of IgM, IgG2a and IgG1 antibody reactivity against potato apyrase were observed in S. mansoni-infected BALB/c mice during the acute phase of infection, while only IgM and IgG1 antibody reactivity levels maintained elevated during the chronic phase of infection. Antibody reactivity against potato apyrase was monitored over an 11-month period in chronically-infected mice treated with oxamniquine. Eleven months later, the level of seropositive IgM decreased significantly (~30%) compared to the level found in untreated, infected mice. The level of seropositive IgG1 decreased significantly four months after treatment (MAT) (61%) and remained at this level even after 11 months. The IgG2a reactivity against potato apyrase, although unchanged during chronic phase to 11 MAT, appeared elevated again in re-infected mice suggesting a response similar to that found during the acute phase. BALB/c mouse polyclonal anti-potato apyrase IgG reacted with soluble egg antigens probably due to the recognition of parasite ATP diphosphohydrolase. This study, for the first time, showed that the IgG2a antibody from S. mansoni-infected BALB mice cross-reacts with potato apyrase and the level of IgG2a in infected mice differentiates disease phases. The results also suggest that different conserved-epitopes contribute to the immune response in schistosomiasis.

【 授权许可】

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