Comparison of HPV genotyping by type-specific PCR and sequencing

Memórias do Instituto Oswaldo Cruz

Nara De Oliveira Carvalho¹  Dora Méndez Del Castillo¹  Carlos Perone¹  José Nélio Januário¹  Victor Hugo De Melo²  Geraldo Brasileiro Filho¹ 

[1] ,Centro de Treinamento e Referência em Doenças Infecciosas e Parasitárias Orestes Diniz

关键词: HPV;    PCR;    sequencing;    typing;   

DOI  :  10.1590/S0074-02762010000100011

来源: SciELO

【 摘 要 】

Human papillomavirus (HPV) infection is the most common sexually transmitted disease worldwide and there is a strong link between certain high-risk viral types and cervical carcinogenesis. Although there are several typing methods, it is still unclear which test is the best. This study compared the effectiveness of type-specific PCR (TS-PCR) and sequencing, with a focus on their clinical application. A total of 260 cervical samples from HPV-positive patients were tested for types 6, 11, 16, 18, 31, 33 and 35 using TS-PCR and sequencing. The genotype was identified in 36% of cases by TS-PCR and in 75% by sequencing. Sequencing was four times more likely to identify the viral type in positive samples than TS-PCR (p = 0.00). Despite being more effective for virus genotyping, sequencing was unable to identify viral types in multiple infections. Combining both techniques resulted in highly sensitive detection (87% of cases), showing that they are complementary methods. HPV genotyping is an important step in HPV management, helping to identify patients with a higher risk of developing cervical cancer and contributing to the development of type-specific vaccines.

【 授权许可】

CC BY   
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