Memórias do Instituto Oswaldo Cruz | |
Comparison of HPV genotyping by type-specific PCR and sequencing | |
Nara De Oliveira Carvalho1  Dora Méndez Del Castillo1  Carlos Perone1  José Nélio Januário1  Victor Hugo De Melo2  Geraldo Brasileiro Filho1  | |
[1] ,Centro de Treinamento e Referência em Doenças Infecciosas e Parasitárias Orestes Diniz | |
关键词: HPV; PCR; sequencing; typing; | |
DOI : 10.1590/S0074-02762010000100011 | |
来源: SciELO | |
【 摘 要 】
Human papillomavirus (HPV) infection is the most common sexually transmitted disease worldwide and there is a strong link between certain high-risk viral types and cervical carcinogenesis. Although there are several typing methods, it is still unclear which test is the best. This study compared the effectiveness of type-specific PCR (TS-PCR) and sequencing, with a focus on their clinical application. A total of 260 cervical samples from HPV-positive patients were tested for types 6, 11, 16, 18, 31, 33 and 35 using TS-PCR and sequencing. The genotype was identified in 36% of cases by TS-PCR and in 75% by sequencing. Sequencing was four times more likely to identify the viral type in positive samples than TS-PCR (p = 0.00). Despite being more effective for virus genotyping, sequencing was unable to identify viral types in multiple infections. Combining both techniques resulted in highly sensitive detection (87% of cases), showing that they are complementary methods. HPV genotyping is an important step in HPV management, helping to identify patients with a higher risk of developing cervical cancer and contributing to the development of type-specific vaccines.
【 授权许可】
CC BY
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
【 预 览 】
Files | Size | Format | View |
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RO202005130047746ZK.pdf | 365KB | download |