Comparative study of two extraction methods for enteric virus recovery from sewage sludge by molecular methods

Memórias do Instituto Oswaldo Cruz

Ad Schlindwein¹  Cmo Simões²  Crm Barardi¹ 

[1] ,Universidade Federal de Santa Catarina Laboratório de Virologia Aplicada Departamento de Ciências FarmacêuticasFlorianópolis SC ,Brasil

关键词: sewage sludge;    virus detection;    RT-nested-PCR;   

DOI  :  10.1590/S0074-02762009000400007

来源: SciELO

【 摘 要 】

The aim of this study was to compare two nucleic acid extraction methods for the recovery of enteric viruses from activated sludge. Test samples were inoculated with human adenovirus (AdV), hepatitis A virus (HAV), poliovirus (PV) and rotavirus (RV) and were then processed by an adsorption-elution-precipitation method. Two extraction methods were used: an organic solvent-based method and a silica method. The organic-based method was able to recoup 20% of the AdV, 90% of the RV and 100% of both the PV and HAV from seeded samples. The silica method was able to recoup 1.8% of the AdV and 90% of the RV. These results indicate that the organic-based method is more suitable for detecting viruses in sewage sludge.

【 授权许可】

CC BY   
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