Distribution of Hepatitis C virus (HCV) genotypes in seropositive patients in the state of Alagoas, Brazil

Brazilian Journal of Microbiology

Rosa Maria S. Gonzaga²  Itatiana F. Rodart¹  Mitermayer Galvão Reis¹  Cícero Eduardo Ramalho Neto²  Denise Wanderlei Silva¹ 

[1] ,Universidade Federal de Alagoas Genômica e Proteômica Laboratório de Genética MolecularMaceió AL ,Brasil

关键词: Hepatitis C virus;    5'NCR;    core region;    RT-nested-PCR;    genotypes;    Vírus da hepatite C;    5'NCR;    região core;    RT-PCR aninhada;    genótipos;   

DOI  :  10.1590/S1517-83822008000400008

来源: SciELO

【 摘 要 】

We determined the frequency of hepatitis C virus (HCV) genotypes in anti-HCV seropositive patients in the state of Alagoas, Brazil, by means of nested-reverse transcription-polymerase chain reaction (RT-nested-PCR) followed by restriction fragment length polymorphism (RFLP) of amplified fragments of the 5´NCR. The nested-PCR with genotype-specific primers from the core region was carried out when detection was not possible by the first approach. Detectable HCV-RNA was present in 115 (74.7%) of 154 serum samples. Genotype 1 was the most frequent (77.4%), against 20.9% of genotype 3 and 0.8% of genotype 2. Subtype 1b was predominant (65.2%), followed by subtypes 1a (8.7%), and 3a (6.1%). Coinfection (1a/3a) was detected in 0.8% of the samples. Indeed, there was no significant differences in the prevalence of genotype 1 compared to what has been obtained from anti-HCV seropositive patients from other locations in Brazil. Here we report for the first time the genotype 2 in the state of Alagoas.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License