Experimental reinfection of BALB/c mice with different recombinant type I/III strains of Toxoplasma gondii: involvement of IFN-³ and IL-10

Memórias do Instituto Oswaldo Cruz

Geane Peroni Brandão¹  Maria Norma Melo¹  Ricardo Tostes Gazzinelli²  Braulia Costa Caetano²  Adriana Melo Ferreira¹  Letícia Azevedo Silva¹  Ricardo Wagner Almeida Vitor¹ 

[1] ,Universidade Federal de Minas Gerais Instituto de Ciências Biológicas Departamento de Bioquímica e ImunologiaBelo Horizonte MG ,Brasil

关键词: experimental toxoplasmosis;    reinfection;    recombinant strains;    cytokine;   

DOI  :  10.1590/S0074-02762009000200017

来源: SciELO

【 摘 要 】

To assess reinfection of BALB/c mice with different Toxoplasma gondii strains, the animals were prime infected with the non-virulent D8 strain and challenged with virulent recombinant strains. Thirty days after challenge, brain cysts were obtained from surviving BALB/c mice and inoculated in Swiss mice to obtain tachyzoites for DNA extraction and PCR-RFLP analysis to distinguish the different T. gondii strains present in possible co-infections. Anti-Toxoplasma immune responses were evaluated in D8-primed BALB/c mice by detecting IFN-³ and IL-10 produced by T cells and measuring immunoglobulin levels in serum samples. PCR-RFLP demonstrated that BALB/c mice were reinfected with the EGS strain at 45 days post prime infection (dpi) and with the EGS and CH3 strains at 180 dpi. High levels of IFN-³ were detected after D8 infection, with no significant difference between 45 and 180-day intervals. However, higher IL-10 levels and higher plasmatic IgG1 and IgA were detected from samples obtained 180 days after infection. BALB/c mice were susceptible to reinfection with different recombinant T. gondii strains and this susceptibility correlated with enhancement of IL-10 production.

【 授权许可】

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