Vanillin production by recombinant strains of Escherichia coli

Brazilian Journal of Microbiology

Attilio Converti²  Danilo De Faveri²  Patrizia Perego²  Paolo Barghini¹  Maurizio Ruzzi¹  Luciane Sene¹ 

[1] ,Universidade de Genova Departamento de Engenharia Química e de Processo Genova,Itália

关键词: vanillin;    ferulate;    Escherichia coli JM109;    recombinant strains;    vanilina;    ácido ferúlico;    Escherichia coli JM109;    linhagens recombinantes;   

DOI  :  10.1590/S1517-83822003000500037

来源: SciELO

【 摘 要 】

Vanillin production from ferulate was studied using different recombinant strains of Escherichia coli. To prevent the occurrence of aerobic conditions and then possible product oxidation, tests were performed in Erlenmeyer flasks under mild mixing (150 rpm). Among other transformants, E. coli JM109(pBB1) appeared to be the best vanillin producer, being able to convert no less than 95% of starting ferulate to the product within 1h. This yield decreased down to 72% after 72h, likely because of a non-specific oxidase activity responsible for vanillin oxidation to vanillate.

【 授权许可】

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