The redox potential interferes with the expression of laminin binding molecules in Bacteroides fragilis

Memórias do Instituto Oswaldo Cruz

Eliane De Oliveira Ferreira²  Edwin Alexander Yates¹  Morris Goldner¹  Rossiane Cláudia Vommaro¹  Fernando Costa E Silva Filho¹  Débora Barreiros Petrópolis¹  Regina Mc Pilotto Domingues² 

[1] ,IMPPG Departamento de Microbiologia Médica Laboratório de Biologia de Anaeróbios

关键词: Bacteroides fragilis;    adhesion;    redox potential;    laminin binding proteins;    outer membrane proteins;   

DOI  :  10.1590/S0074-02762008000700010

来源: SciELO

【 摘 要 】

The Bacteroides fragilis ATCC strain was grown in a synthetic media with contrasting redox potential (Eh) levels [reduced (-60 mV) or oxidised (+100mV)] and their adhesion capacity to extracellular matrix components was evaluated. The strain was capable of adhering to laminin, fibronectin, fibronectin + heparan sulphate and heparan sulphate. A stronger adherence to laminin after growing the strain under oxidising conditions was verified. Electron microscopy using ruthenium red showed a heterogeneous population under this condition. Dot-blotting analyses confirmed stronger laminin recognition by outer membrane proteins of cells cultured at a higher Eh. Using a laminin affinity column, several putative laminin binding proteins obtained from the cultures kept under oxidising (60 kDa, 36 kDa, 25 kDa and 15 kDa) and reducing (60 kDa) conditions could be detected. Our results show that the expression of B. fragilis surface components that recognise laminin are influenced by Eh variations.

【 授权许可】

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