Genetic characterization of morphologically variant strains of Paracoccidioides brasiliensis

Memórias do Instituto Oswaldo Cruz

Cintia De Moraes Borba²  Janaina Correia¹  Ester Vinhas¹  Albert Martins¹  Beatriz Costa Aguiar Alves¹  Sheila Unkles¹  James Robertson Kinghorn¹  Norma Lucena-silva¹ 

[1] ,Fiocruz Instituto Oswaldo Cruz Departamento de MicologiaRio de Janeiro RJ ,Brasil

关键词: FMO;    Paracoccidioides brasiliensis;    RAPD-PCR;   

DOI  :  10.1590/S0074-02762008005000013

来源: SciELO

【 摘 要 】

Molecular characterization of Paracoccidioides brasiliensis variant strains that had been preserved under mineral oil for decades was carried out by random amplified polymorphic DNA analysis (RAPD). On P. brasiliensis variants in the transitional phase and strains with typical morphology, RAPD produced reproducible polymorphic amplification products that differentiated them. A dendrogram based on the generated RAPD patterns placed the 14 P. brasiliensis strains into five groups with similarity coefficients of 72%. A high correlation between the genotypic and phenotypic characteristics of the strains was observed. A 750 bp-RAPD fragment found only in the wild-type phenotype strains was cloned and sequenced. Genetic similarity analysis using BLASTx suggested that this RAPD marker represents a putative domain of a hypothetical flavin-binding monooxygenase (FMO)-like protein of Neurospora crassa.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License