Inhibition of 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine metabolic activity of porcine FAD‐containing monooxygenase by selective monoamine oxidase‐B inhibitors

FEBS Letters

Ichikawa, Yoshiyuki¹  Wu, Ru-Feng¹ 

[1] Department of Biochemistry, Kagawa Medical School, Kita-gun, Kagawa 761-07, Japan

关键词: FAD-containing monooxygenase;    Monoamine oxidase-B inhibitor;    Deprenyl;    Pargyline;    FMO;    FAD-containing monooxygenase;    MAO;    monoamine oxidase;    MPTP;    1-methyl-4-phenyl-1;    2;    3;    6-tetrahydropyridine;   

DOI  :  10.1016/0014-5793(94)01412-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The MPTP metabolic activity of porcine FAD-containing monooxygenase (FMO) (EC 1.14.13.8) was inhibited considerably by deprenyl and pargyline, selective MAO-B inhibitors, and they showed typical competitive inhibition. Deprenyl and pargyline, amine derivatives were also examined as to whether they are substrates for the FMO. It was found that deprenyl and pargyline are excellent substrates for the FMO. The K _i and K _m values of deprenyl and pargyline for the FMO are 14 μM and 9 μM, and 14.3 μM and 11.6 μM, at pH 8.0 and 25°C, respectively.

【 授权许可】

Unknown