Immunological and microbiological activity of Davilla elliptica St. Hill. (Dilleniaceae) against Mycobacterium tuberculosis

Memórias do Instituto Oswaldo Cruz

Flávia Cristine Mascia Lopes²  Marisa Campos Polesi Placeres²  Cleso Mendonça Jordão Junior²  Célio Takashi Higuchi¹  Daniel Rinaldo¹  Wagner Vilegas¹  Clarice Queico Fujimura Leite¹  Iracilda Zeppone Carlos² 

[1] ,Unesp Departamento de Análises Clínicas

关键词: Davilla elliptica;    antimycobacterial activity;    immunological activity;   

DOI  :  10.1590/S0074-02762007005000086

来源: SciELO

【 摘 要 】

Mycobacterium tuberculosis is responsible for over 8 million cases of tuberculosis (TB) annually. Natural products may play important roles in the chemotherapy of TB. The immunological activity of Davilla elliptica chloroform extract (DECE) was evaluated in vitro by the determination of hydrogen peroxide (H2O2), nitric oxide (NO), and tumor necrosis factor-alpha (TNF-alpha) release in peritoneal macrophages cultures. DECE was also tested for its antimycobacterial activity against M. tuberculosis using the microplate alamar blue assay. DECE (50, 150, 250 µg/ml) stimulated the production of H2O2 (from 1,79 ± 0,23 to 7,27 ± 2,54; 15,02 ± 2,86; 20,5 ± 2,1 nmols) (means ± SD), NO (from 2,64 ± 1,02 to 25,59 ± 2,29; 26,68 ± 2,41; 29,45 ± 5,87 µmols) (means ± SD) and TNF-alpha (from 2,44 ± 1,46 to 30,37 ± 8,13; 38,68 ± 1,59; 41,6 ± 0,90 units/ml) (means ± SD) in a dose-dependent manner and also showed a promising antimycobacterial activity with a minimum inhibitory concentration of 62,5 µg/ml. This plant may have therapeutic potential in the immunological and microbiological control of TB.

【 授权许可】

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