Use of the paired samples (cerebrospinal fluid and serum) in immunodiagnostic of active and inactive human neurocysticercosis

Memórias do Instituto Oswaldo Cruz

Ivanildes Solange Da Costa Barcelos²  Marcelo Simão Ferreira¹  Leandro Pajuaba De Moura¹  Germano Francisco Biondi¹  Julia Maria Costa-cruz² 

[1] ,Universidade Federal de Uberlândia Instituto de Ciências Biomédicas Laboratório de Parasitologia

关键词: Taenia crassiceps;    neurocysticercosis;    enzyme-linked immunosorbent assay;    serum;    cerebrospinal fluid;   

DOI  :  10.1590/S0074-02762005000400014

来源: SciELO

【 摘 要 】

Paired samples of cerebrospinal fluid (CSF) and serum of 30 patients - 10 with active, 10 with inactive neurocysticercosis (NCC), and 10 control subjects - were evaluated by enzyme-linked immunosorbent assay (ELISA) using two Taenia crassiceps metacestode extracts as antigen in order to detect IgG antibodies. In active NCC, high levels of IgG were detected (p < 0.05). The CSF samples showed 80% (CI 72-88) of reactivity in the saline extract (S) and 90% (CI 84-95) in sodium dodecyl sulphate (SDS) and the serum samples were reactive in 90% (CI 84-95) and 100% (CI 98-100) in the S and SDS antigenic extracts, respectively. The use of the paired samples of CSF and serum in active NCC showed equivalent results suggesting that the serum samples could be used as a screening in those patients whose CSF puncture is counter-indicated.

【 授权许可】

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