Detection of Cryptosporidium - specific coproantigen in human immunodeficiency virus/acquired immunodeficiency syndrome patients by using a commercially available immunoenzymatic assay

Memórias do Instituto Oswaldo Cruz

Claudio Vieira Silva²  Marcelo Simão Ferreira¹  Maria Do Rosário De Fátima Gonçalves-pires²  Julia Maria Costa-cruz² 

[1] ,Instituto de Ciências Biomédicas Laboratório de Parasitologia

关键词: Cryptosporidium sp.;    coproantigen;    acquired immunodeficiency syndrome (AIDS);   

DOI  :  10.1590/S0074-02762003000800022

来源: SciELO

【 摘 要 】

The aim of this study was to verify the occurrence of Cryptosporidium infection in 52 human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) patients (group 1) and 38 clinically healthy individuals (group 2) by using enzyme immunoassay (EIA). All fecal samples collected were submitted to the Baermann, Lutz, and Ritchie methods, the Safranin/Methylene Blue, and Weber's chromotrope modified Trichrome staining techniques, and EIA. In group 1, parasitological staining techniques and EIA were both positive for Cryptosporidium sp. infection in 3/52 (5.8%) samples and both negative in 45/52 (86.5%) samples, while 4/52 (7.7%) samples were positive in EIA and negative in parasitological staining techniques. Concerning group 2, all samples were negative by EIA and microscopy for Cryptosporidium infection. In conclusion, EIA may be an alternative method for detecting Cryptosporidium-specific coproantigen in HIV/AIDS patients.

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