Flow cytometry as a tool to identify Mycobacterium tuberculosis interaction with the immune system and drug susceptibility

Memórias do Instituto Oswaldo Cruz

Maria Da Gloria Bonecini-almeida¹ 

[1] ,Fundação Oswaldo Cruz Centro de Pesquisa Hospital Evandro Chagas Departamento de Microbiologia e ImunologiaRio de Janeiro RJ ,Brasil

关键词: tuberculosis;    macrophages;    drug susceptibility;    cytometry;   

DOI  :  10.1590/S0074-02762000000400008

来源: SciELO

【 摘 要 】

Flow cytometric analysis is a useful and widely employed tool to identify immunological alterations caused by different microorganisms, including Mycobacterium tuberculosis. However, this tool can be used for several others analysis. We will discuss some applications for flow cytometry to the study of M. tuberculosis, mainly on cell surface antigens, mycobacterial secreted proteins, their interaction with the immune system using inflammatory cells recovered from peripheral blood, alveolar and pleura spaces and the influence of M. tuberculosis on apoptosis, and finally the rapid determination of drug susceptibility. All of these examples highlight the usefulness of flow cytometry in the study of M. tuber-culosis infection.

【 授权许可】

CC BY   
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