Comparative evaluation under routine conditions of the nitrate reduction assay, the proportion assay and the MGIT 960 assay for drug susceptibility testing of clinical isolates of Mycobacterium tuberculosis

Memórias do Instituto Oswaldo Cruz

Leila De Souza Fonseca¹  Gisele Betzler De Oliveira Vieira¹  Luciana Fonseca Sobral¹  Elizabete Oliveira Ribeiro¹  Anna Grazia Marsico¹ 

[1] ,Complexo Hospitalar Instituto de Doenças do Tórax Laboratório de Micobacteriologia

关键词: tuberculosis;    nitrate reductase assay;    drug susceptibility;   

DOI  :  10.1590/S0074-02762012000100021

来源: SciELO

【 摘 要 】

The performance of the nitrate reductase assay (NRA) was compared with the proportion method (PM) on Lowenstein-Jensen medium and the BACTEC MGIT960 assay under routine conditions using 160 clinical isolates of Mycobacterium tuberculosis with a high proportion of resistant strains. The mean time to obtain results was 8.8 days and the overall agreements between NRA and PM and NRA and M960 were 95% and 94%, respectively. NRA was easy to perform and represents a useful tool for the rapid screening of drug-resistant M. tuberculosis strains in low-resource countries.

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