Evaluation of a monoclonal antibody affinity purified antigen for zymodeme specific serological diagnosis of Trypanosoma cruzi infection

Memórias do Instituto Oswaldo Cruz

Mauro Schechter¹ 

[1] ,London School of Hygiene and Tropical Medicine Department of Medical Protozoology Wolfson Molecular Biology UnitLondon,s.p

关键词: Trypanosoma cruzi;    zymodemes;    diagnosis;    monoclonal antibodies;    Trypanosoma cruzi;    zimodemas;    diagnóstico;    anticorpos monoclonais;   

DOI  :  10.1590/S0074-02761987000100014

来源: SciELO

【 摘 要 】

Theoretically, serological assays with affinity purified marker antigens can allow strain-specific diagnosis even when parasites cannot be retrieved from and infected host. A Trypanosoma cruzi antigen was purified by affinity chromatography using a zymodeme (Z) 2 specific monoclonal antibody (2E2C11). An indirect enzyme-linked immunosorbent assay (ELISA) based on the purified antigen could discriminate between sera from rabbits immunized with T. cruzi zymodeme clones but could not discriminate between sera from mice infected with different zymodemes.

【 授权许可】

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