Isoenzyme profiles of Trypanosoma cruzi stocks from different areas of Paraguay

Memórias do Instituto Oswaldo Cruz

Nidia Acosta²  Margarita Samudio²  Elsa López²  Fernando Vargas¹  Nina Yaksic¹  Simone Frédérique Brenière¹  Antonieta Rojas De Arias² 

[1] ,UNA Instituto de Investigaciones en Ciencias de la Salud Departamento de Medicina TropicalAsunción,Paraguay

关键词: Trypanosoma cruzi;    zymodemes;    genetic polymorphism;    clonality;    Paraguay;   

DOI  :  10.1590/S0074-02762001000400015

来源: SciELO

【 摘 要 】

Twenty one Trypanosoma cruzi stocks from humans, domiciliary triatomines and one sylvatic animal of different areas of Paraguay were subjected to isoenzyme analysis. Thirteen enzyme systems (15 loci in total) were studied. MN cl2 (clonets 39) and SO34 cl4 (clonets 20) were used as references. Relationships between stocks were depicted by an UPGMA dendrogram constructed using the Jaccard´s distances matrix. Among the Paraguayan stocks 14 zymodemes were identified (Par1 to Par14), Par 5 being the most frequent. Polymorphism rate and clonal diversity were 0.73 and 0.93, respectively. Average number of alleles per polymorphic locus was 2.5 (range 2-4). These measurements show a high diversity, which is confirmed by the dendrogram topology. All stocks belong to the same lineage, as MN cl2 reference strain (T. cruzi II). Moreover three distinct subgroups were identified and two of them correspond to Brazilian and Bolivian zymodemes, respectively. The third subgroup, the most common in Paraguay, is related to Tulahuen stock. The large geographical distribution of some zymodemes agrees with the hypothesis of clonality for T. cruzi populations. However sample size was not adequate to detect genetic recombination in any single locality.

【 授权许可】

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