Molecular identification of the agent of Q fever – Coxiella burnetii – in domestic animals in State of Rio de Janeiro, Brazil

Revista da Sociedade Brasileira de Medicina Tropical

Maria Angélica Monteiro De Mello Mares-guia¹  Tatiana Rozental¹  Alexandro Guterres¹  Raphael Gomes¹  Daniele Nunes De Almeida¹  Namir Santos Moreira¹  Jairo Dias Barreira¹  Alexsandra Rodrigues Favacho¹  Andrea Lopes Santana¹  Elba Regina Sampaio De Lemos¹ 

关键词: Q fever;    Coxiella burnetii;    Domestic animals;    Molecular diagnosis;    Brazil;   

DOI  :  10.1590/0037-8682-0076-2013

来源: SciELO

【 摘 要 】

Introduction Over the last recent years, the number of Q fever cases have has increased throughout the world. An epidemiological investigation was performed in the area in which the first molecular documentation of Q fever in Brazil was previously reported. Methods Indirect immunofluorescence assay (IFA) and PCR of Coxiella burnetii targeting the htpAB gene were performed in samples from 14 dogs (blood); 1 cat (blood); 10 goats (blood, milk, vaginal swab and anal swab); 3 sheep (blood); and 2 horses (blood). Results Two dogs, two sheep and five goats were seroreactive. DNA was amplified from 6 milk and 2 blood samples from goats and from dogs, respectively. The sequence of the amplicons exhibited 99% sequence similarity with the homologous sequence of the htpAB gene of C. burnetii RSA 331 (GenBank - CP000890). Conclusions The results confirm C. burnetii infection in animals in Rio de Janeiro and reinforce the need for the surveillance of Q fever in Brazil.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License