Monitoring Trypanosoma cruzi infection in triatomines using PCR in Mato Grosso do Sul, Brazil

Revista da Sociedade Brasileira de Medicina Tropical

Marlon Cezar Cominetti¹  Robson Ferreira Cavalcante De Almeida¹  Guilmara Maria Do Amaral Goncalves¹  Renato Andreotti¹ 

关键词: Epidemiology;    Triatomine infection monitoring;    Polymerase chain reaction;    Microscopy examination;    Trypanosoma cruzi;   

DOI  :  10.1590/0037-8682-0024-2013

来源: SciELO

【 摘 要 】

Introduction The aim of the present study was to assess the polymerase chain reaction (PCR) as a method for detecting Trypanosoma cruzi infection in triatomines that had been previously determined by microscopic examination in the State of Mato Grosso do Sul, Brazil. Methods In total, 515 specimens were collected. Material from the digestive tract of each triatomine was analyzed for the presence of T. cruzi by microscopic examination and PCR using the 121/122 primer set. Results Among the 515 specimens tested, 58 (11.3%) were positive by microscopy and 101 (19.61%) were positive by PCR and there was an association between the results of the techniques (χ2 = 53.354, p = 0.001). The main species of triatomine identified was T. sordida (95.5%) Conclusions The use of PCR in entomological surveillance may contribute to a better assessment of the occurrence of T. cruzi in triatomine populations.

【 授权许可】

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