Sharing of antigens between Plasmodium falciparum and Anopheles albimanus

Revista do Instituto de Medicina Tropical de São Paulo

Albina Wide¹  Jacinta Capaldo²  Noraida Zerpa²  Rosalba Pabon²  Alfredo Noda²  Belkis Noya¹  Julio González²  Oscar Noya¹ 

[1] ,Universidad Central de Venezuela Facultad de Medicina Instituto de Medicina TropicalCaracas,Venezuela

关键词: Anopheles albimanus;    Plasmodium falciparum;    Shared antigens;    Cross-reaction;    Multiple Antigen Blot Assay (MABA);   

DOI  :  10.1590/S0036-46652006000600005

来源: SciELO

【 摘 要 】

The presence of common antigens between Plasmodium falciparum and Anopheles albimanus was demonstrated. Different groups of rabbits were immunized with: crude extract from female An. albimanus (EAaF), red blood cells infected with Plasmodium falciparum (EPfs), and the SPf66 synthetic malaria vaccine. The rabbit's polyclonal antibodies were evaluated by ELISA, Multiple Antigen Blot Assay (MABA), and immunoblotting. All extracts were immunogenic in rabbits according to these three techniques, when they were evaluated against the homologous antigens. Ten molecules were identified in female mosquitoes and also in P. falciparum antigens by the autologous sera. The electrophoretic pattern by SDS-PAGE was different for the three antigens evaluated. Cross-reactions between An. albimanus and P. falciparum were found by ELISA, MABA, and immunoblotting. Anti-P. falciparum and anti-SPf66 antibodies recognized ten and five components in the EAaF crude extract, respectively. Likewise, immune sera against female An. albimanus identified four molecules in the P. falciparum extract antigen. As far as we know, this is the first work that demonstrates shared antigens between anophelines and malaria parasites. This finding could be useful for diagnosis, vaccines, and the study of physiology of the immune response to malaria.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License