Neurocryptococcosis: diagnosis by PCR method

Revista do Instituto de Medicina Tropical de São Paulo

Regina Célia Paschoal²  Mário Hiroyuki Hirata²  Rosário Crespo Hirata²  Márcia De Souza Carvalho Melhem¹  Amanda Latercia Tranches Dias¹  Claudete Rodrigues Paula¹ 

[1] ,University of São Paulo Faculty of Pharmaceutical Sciences Department of Clinical Analyses and ToxicologySão Paulo SP ,Brazil

关键词: Cryptococcus neoformans;    PCR;    Diagnostic methods;    AIDS;    Cryptococcosis;   

DOI  :  10.1590/S0036-46652004000400006

来源: SciELO

【 摘 要 】

Cryptococcus neoformans detection was optimized using PCR technique with the objective of application in the clinical laboratory diagnosis. The amplification area was ITS and 5,6S which encodes the ribosomal RNA (rRNA). A total of 72 cerebrospinal fluid (CSF) samples were used, obtained from cases with and without AIDS. The patients had cryptococcal meningitis (n = 56) and meningitis caused by other agents (n = 16). The results demonstrated that PCR test had the highest sensitivity rates, superior to culture (85.7%) and to India ink test (76.8%). PCR was found to be sensitive in detecting 1 cell/mL and highly specific since it did not amplify other fungal DNA. The comparative analysis of the methods showed that PCR is more sensitive and specific and is applicable as an important laboratorial resource for neurocryptococcosis diagnosis.

【 授权许可】

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