Revista do Instituto de Medicina Tropical de São Paulo | |
Molecular characterization of Dengue viruses type 1 and 2 isolated from a concurrent human infection | |
Cecília Luiza Simões Dos Santos2  Mariana Aparecida Antunes Bastos2  Maria Anice Mureb Sallum1  Iray Maria Rocco2  | |
[1] ,Instituto Adolfo Lutz Serviço de Virologia São Paulo SP ,Brasil | |
关键词: Dengue virus; Dual infection; RT/PCR; E/NS1 gene sequences; Phylogenetic analysis; | |
DOI : 10.1590/S0036-46652003000100003 | |
来源: SciELO | |
【 摘 要 】
In 2001, an autochthonous case of dual viremia, resulting from naturally acquired dengue virus DEN-1 and DEN-2 infections was detected during the dengue outbreak that occurred in Barretos, a city with about 105,000 inhabitants in the North region of São Paulo State. Serotype identification was based on virus isolation to C6/36 mosquito cells culture and immunofluorescence assays using type-specific monoclonal antibodies. The double infection was also confirmed by reverse transcriptase polymerase chain reaction (RT-PCR). Comparative analysis of the 240-nucleotide sequences of E/NS1 gene junction region between the genome of DEN-1 and DEN-2 isolates of the corresponding reference Nauru and PR 159S1 strains, respectively, showed some nucleotide differences, mainly silent mutations in the third codon position. Results of maximum likelihood phylogenetic analysis of E/NS1 gene sequences indicated that both genotypes of DEN-1 and DEN-2 viruses recovered from double infection in Barretos belonged to genotypes I and III, respectively.
【 授权许可】
CC BY-NC
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202103040033060ZK.pdf | 762KB | download |