Molecular characterization of Dengue viruses type 1 and 2 isolated from a concurrent human infection

Revista do Instituto de Medicina Tropical de São Paulo

Cecília Luiza Simões Dos Santos²  Mariana Aparecida Antunes Bastos²  Maria Anice Mureb Sallum¹  Iray Maria Rocco² 

[1] ,Instituto Adolfo Lutz Serviço de Virologia São Paulo SP ,Brasil

关键词: Dengue virus;    Dual infection;    RT/PCR;    E/NS1 gene sequences;    Phylogenetic analysis;   

DOI  :  10.1590/S0036-46652003000100003

来源: SciELO

【 摘 要 】

In 2001, an autochthonous case of dual viremia, resulting from naturally acquired dengue virus DEN-1 and DEN-2 infections was detected during the dengue outbreak that occurred in Barretos, a city with about 105,000 inhabitants in the North region of São Paulo State. Serotype identification was based on virus isolation to C6/36 mosquito cells culture and immunofluorescence assays using type-specific monoclonal antibodies. The double infection was also confirmed by reverse transcriptase polymerase chain reaction (RT-PCR). Comparative analysis of the 240-nucleotide sequences of E/NS1 gene junction region between the genome of DEN-1 and DEN-2 isolates of the corresponding reference Nauru and PR 159S1 strains, respectively, showed some nucleotide differences, mainly silent mutations in the third codon position. Results of maximum likelihood phylogenetic analysis of E/NS1 gene sequences indicated that both genotypes of DEN-1 and DEN-2 viruses recovered from double infection in Barretos belonged to genotypes I and III, respectively.

【 授权许可】

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