A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp

Revista do Instituto de Medicina Tropical de São Paulo

Francisco Hernández¹ 

[1] ,University of Costa Rica Faculty of Microbiology San José,Costa Rica

关键词: Campylobacter;    technical assay;    Microaerobiosis;   

DOI  :  10.1590/S0036-46651996000300015

来源: SciELO

【 摘 要 】

Faeces of 138 chickens were inoculated on Blaser agar plates. One set of plates was incubated in jars with CampyPak envelopes. The others were incubated in "Zip-lock" plastic bags (7 x X in.) and a microaerophilic atmosphere was generated exhaling into the "Zip-lock" plastic bag, after holding the breath for 20 sec. Then, the bag was pressed to evacuate its atmosphere, inflated again, and pressed (4 times), and finally sealed. Campylobacter was isolated from 127 (96.2%) of samples incubated in jars with gas generator envelopes and from 129 (98%) of the specimens incubated into the bags. The proposed methodology offers good savings for cost-conscious laboratories.

【 授权许可】

CC BY-NC   
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