A low cost method to produce a gaseous environment for the isolation of Helicobacter pylori

Revista do Instituto de Medicina Tropical de São Paulo

Francisco Hernández²  Patricia Rivera¹ 

[1] ,Universidad de Costa Rica Facultad de Microbiología Departamento de MicrobiologíaSan José,Costa Rica

关键词: Helicobacter pylori;    Technical assay;    Microaerobiosis;   

DOI  :  10.1590/S0036-46651992000200013

来源: SciELO

【 摘 要 】

A low cost method (LCM) to produce a gaseous environment for the isolation of Helicobacter pylori, was compared with the standard Gas Park system. The LCM uses a carbonated antacid tablet, a plastic bag with tap water, a candle, and a wide-mouthed glass jar provided with a tight-fitting metalic screw cap and a rubber gasket. Antral gastric biopsies from 153 cases were incubated by duplicate on blood agar plates and treated with the two methods. In 95 cases the agent was isolated from both, and only from the standard method in 10 cases; the opposite condition was found in five cases, and 43 were negative. That difference is not significant (Pearson's X²= 93.25 p > 0,05)

【 授权许可】

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