Entamoeba histolytica: fecal antigen capture immunoassay for the diagnosis of enteric amebiasis by a monoclonal antibody

Revista do Instituto de Medicina Tropical de São Paulo

Haydeé Urdaneta²  Antonio Rangel²  Maria Sonia Martins¹  Jose Francisco Muñoz²  Manuel Hernández M² 

[1] ,Los Andes University, Venezuela Institute of Clinical Immunology ,Venezuela

关键词: Entamoeba histolytica;    Amebiasis;    Immunodiagnostic;    Monoclonal antibodies;    Capture ELISA;   

DOI  :  10.1590/S0036-46651996000100008

来源: SciELO

【 摘 要 】

Amebiasis continues to be of epidemiological importance in underdeveloped countries. Clinical diagnosis and epidemiological setting in a region are based on the fecal microscopic identification of cysts or trophozoites. This procedure requires well trained personnel, is laborious, of low sensitivity and frequently yields false-positives results. The present study was designed to develop an immuno-enzymatic fecal 96 kDa antigen capture test (COPROELISA-Eh) more sensitive and specific than microscopic diagnosis of amebiasis. Triplicates of 177 stool samples processed by the formol-ether concentration method, were defined as positive or negative by three experienced microscopic observers. Another aliquot was submitted to the antigen capture test by a monoclonal antibody against a specific membrane antigen of pathogenic strains of Entamoeba histolytica. Optical densities were interpreted as positive when they exceeded the mean value of negative samples plus two standard deviations. COPROELISA-Eh showed a 94.4% sensitivity, 98.3% specificity, 96.2% positive predictive value and 97.6% negative predictive value for the detection of E. histolytica in feces. COPROELISA-Eh is more sensitive and specific than microscopic examination, does not require specially trained personnel and allows the simultaneous processing of a large number of samples.

【 授权许可】

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