Entamoeba histolytica: detection of coproantigens by purified antibody in the capture sandwich ELISA

Revista do Instituto de Medicina Tropical de São Paulo

Haidee Urdaneta²  Semíramis Guimarães¹  Edward F. Silva¹  Carlos A. P. Tavares¹ 

[1] ,Los Andes University Department of Biology

关键词: Entamoeba histolytica;    Amebiasis;    Coproantigens;    Immunodiagnostic;    Purified antibody;    Capture Sandwich ELISA (CSE);   

DOI  :  10.1590/S0036-46651994000600011

来源: SciELO

【 摘 要 】

A sensitive and specific Capture Sandwich ELISA (CSE) was developed using polyclonal purified rabbit antibodies against three different axenic strains of Entamoeba histolytica: CSP from Brazil and HM1 - IMSS from Mexico, for the detection of coproantigens in fecal samples. Immunoglobulin G (IgG) againstis E. histolytica was isolated from rabbits immunized with throphozoites whole extract in two stages: affinity chromatography in a column containing E. histolytica antigens bound to Sepharose 4B was followed by another chromatography in Sepharose antibodies 4B-Protein A. A Capture Sandwich ELISA using purified antibodies was able to detect 70ng of amebae protein, showing a sensitivity of 93% and specificity of 94%. The combination of microscopic examination and CSE gave a concordance and discordance of 93.25% and 6.75%, respectively. It was concluded that CSE is highly specific for the detection of coproantigens of E. histolytica in feces of infected patients, is quicker to perform, easier and more sensitive than microscopic examination.

【 授权许可】

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