Comparative studies of Yersinia pestis outer membrane isolation techniques and their potential use in plague epidemiology

Revista do Instituto de Medicina Tropical de São Paulo

Frederico Guilherme Coutinho Abath²  Luís Carlos De Sousa Ferreira¹ 

[1] ,Fundação Oswaldo Cruz Centro de Pesquisas Aggeu Magalhães Recife Pernambuco ,Brazil

关键词: Yersinia pestis;    outer membrane proteins;    membrane isolation techniques;   

DOI  :  10.1590/S0036-46651990000200002

来源: SciELO

【 摘 要 】

In the present study three techniques for obtaining outer membrane enriched fractions from Yersinia pestis were evaluated. The techniques analysed were: differential solubilization of the cytoplasmic membrane with Sarkosyl or Triton X-100, and centrifugation in sucrose density gradients. The sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of outer membrane isolated by the different methods resulted in similar protein patterns. The measurement of NADH-dehydrogenase and succinate dehydrogenase (inner membrane enzymes) indicated that the outer membrane preparations obtained by the three methods were pure enough for analytical studies. In addition, preliminary evidences on the potential use of outer membrane proteins for the identification of geographic variants of Y. pestis wild isolates are presented.

【 授权许可】

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