Pre, intra and post-ischemic hypothermic neuroprotection in temporary focal cerebral ischemia in rats: morphometric analysis

Arquivos de Neuro-Psiquiatria

Roberto Alexandre Dezena¹  Benedicto Oscar Colli¹  Carlos Gilberto Carlotti Junior¹  Luís Fernando Tirapelli¹ 

[1] ,Universidade de São Paulo Ribeirão Preto Medical School Department of Surgery and AnatomyRibeirão Preto SP ,Brazil

关键词: brain ischemia;    mild hypothermia;    morphometry;    neuroprotection;    reperfusion;    isquemia encefálica;    hipotermia leve;    morfometria;    neuroproteção;    reperfusão;   

DOI  :  10.1590/S0004-282X2012000800010

来源: SciELO

【 摘 要 】

OBJECTIVE: To evaluate the neuroprotection of mild hypothermia, applied in different moments, in temporary focal cerebral ischemia in rats. METHODS: Rats was divided into Control (C), Sham (S), Ischemic-control(IC), Pre-ischemic Hypothermia (IH1), Intra-ischemic Hypothermia (IH2), and Post-ischemic Hypothermia (IH3) groups. Morphometry was performed using the KS400 software (Carl Zeiss®) in coronal sections stained by Luxol Fast Blue. Ischemic areas and volumes were obtained. RESULTS: Statistically, blue areas showed difference for C vs. IC, IC vs. IH1 and IC vs. IH2 (p=0.0001; p=0.01; p=0.03), and no difference between C vs. S, IC vs. IH3 and IH vs. IH2 (p=0.39; p=0.85; p=0.63). Red areas showed difference between C vs. IC, IC vs. IH1 and IC vs. IH2 (p=0.0001; p=0.009; p=0.03), and no difference between C vs. S, IC vs. IH3 and IH1 vs. IH2 (p=0.48; p=0.27; p=0.68). Average ischemic areas and ischemic volumes showed difference between IC vs. IH1 and IC vs. IH2 (p=0.0001 and p=0.0011), and no difference between IC vs. IH3 and IH1 vs. IH2 (p=0.57; p=0.79). CONCLUSION: Pre-ischemic and intra-ischemic hypothermia were shown to be similarly neuroprotective, but this was not true for post-ischemic hypothermia.

【 授权许可】

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