Semi-quantitative analysis of the effects of cyclosporine on remyelination following gliotoxic injection in the brainstem

Arquivos de Neuro-Psiquiatria

E F Bondan²  M F M Martins¹  A M C Branco²  M A Lallo² 

[1] ,University Paulista

关键词: cyclosporine;    central nervous system;    ethidium bromide;    oligodendroglia;    remyelination;    Schwann cells;    semi-quantitative analysis;    análise semiquantitativa;    brometo de etídio;    células de Schwann;    ciclosporina;    oligodendróglia;    remielinização;    sistema nervoso central;   

DOI  :  10.1590/S0004-282X2011000300021

来源: SciELO

【 摘 要 】

The use of cyclosporine (CsA) has shown to induce an increase in the density of oligodendrocytes near remyelinating areas following the injection of ethidium bromide (EB), a demyelinating agent, in the rat brainstem. This study was designed in order to evaluate if CsA has the capacity of increasing remyelination. In this context, a comparison between the final balance of myelin repair in CsA treated and non-treated rats was assessed using a semi-quantitative method developed for documenting the extent and nature of remyelination in gliotoxic lesions. Wistar rats were submitted to intracisternal injection of 10 microliters of 0.1% EB. Some were treated during 31 days with CsA (group III - 10 mg/kg/day by 7 days and, thereafter, 3 times a week, with a minimal interval of 48 hours) by intraperitonial route. Others were not treated with CsA (group I). A control group was planned receiving into the cisterna pontis 10 microliters of 0.9% saline solution and following after that the same CsA administration protocol (group II). Results clearly demonstrate that in vivo administration of CsA after EB-demyelinating lesions stimulated oligodendrocyte remyelination (mean remyelination scores of 3.72±0.25 for oligodendrocytes and 1.04±0.39 for Schwann cells) compared to non-treated animals (3.13±0.71 and 1.31±0.62, respectively), although the mechanisms by which this positive CsA effect occurs are unclear.

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